Nuclear Receptor Reporter Assays in Co-Culture with upcyte® Hepatocytes Reveal Metabolism-Dependent Endocrine Activity of Methoxychlor

Detect metabolism-dependent endocrine activity using metabolically competent hepatocyte co-culture systems.

Many endocrine-active compounds require metabolic activation before they exhibit biological activity. Conventional nuclear receptor reporter assays often lack metabolic competence and may underestimate these effects.

In this SOT 2026 poster, INDIGO Biosciences researchers demonstrate how co-culture with upcyte® human hepatocytes enables detection of metabolism-dependent endocrine activity, using methoxychlor as a model compound.

What You'll Learn:

• How metabolically competent hepatocyte co-cultures enhance nuclear receptor reporter assays
• How methoxychlor metabolism drives ERα activation
• How CYP450 inhibition confirms metabolism-dependent endocrine activity