---
title: Detect metabolism-dependent endocrine activity using metabolically competent hepatocyte co-culture systems | INDIGO Biosciences
---

[![Indigo_Logo_RGB_small](https://info.indigobiosciences.com/hs-fs/hubfs/Indigo_Logo_RGB_small.png?width=197&name=Indigo_Logo_RGB_small.png "Indigo_Logo_RGB_small")](https://indigobiosciences.com/)

# Nuclear Receptor Reporter Assays in Co-Culture with upcyte® Hepatocytes Reveal Metabolism-Dependent Endocrine Activity of Methoxychlor

#### Detect metabolism-dependent endocrine activity using metabolically competent hepatocyte co-culture systems.

Many endocrine-active compounds require metabolic activation before they exhibit biological activity. Conventional nuclear receptor reporter assays often lack metabolic competence and may underestimate these effects.

In this SOT 2026 poster, INDIGO Biosciences researchers demonstrate how **co-culture with upcyte® human hepatocytes enables detection of metabolism-dependent endocrine activity**, using methoxychlor as a model compound.

#### What You'll Learn:

- How **metabolically competent hepatocyte co-cultures enhance nuclear receptor reporter assays**
- How **methoxychlor metabolism drives ERα activation**
- How **CYP450 inhibition confirms metabolism-dependent endocrine activity**

 Download the full poster to see the data and discover how INDIGO's co-culture system can improve detection of endocrine liabilities not observed in traditional reporter assays.

 

##  Get the Full Poster 

Fill out the form below to access the poster instantly!

 

 

 

![](https://px.ads.linkedin.com/collect/?pid=493689&fmt=gif)